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promega，M-MLV Reverse Transcriptase，M1701

描述：上海起發(fā)簽約promega

更新時(shí)間：2015-06-18
訪問次數(shù)：3160
廠商性質(zhì)：代理商
詳情介紹

M-MLV Reverse Transcriptase
·                                 Protocol
·                                 MSDS
·                                 Components
M-MLV Reverse Transcriptase
·                                 M-MLV Reverse Transcriptase
·                                 M-MLV RT 5X Buffer
10,000u
200u/μl
M1701

 
QC Tests
Activity, SDS-PAGE/purity, DNase, RNase, endonuclease, first-strand cDNA synthesis.
Source
Recombinant E. coli strain.
Storage Buffer
20mM Tris-HCl （pH 7.5 at 25°C）, 0.2M NaCl, 0.1mM EDTA, 1mM DTT, 0.01% Nonidet® P-40 and 50% glycerol.
Storage Conditions
Store at –20°C.
Unit Definition
One unit is the amount of enzyme required to incorporate 1nmol of deoxynucleotide into acid-precipitable material in 10 minutes at 37°C. The reaction conditions are: 50mM Tris-HCl （pH 8.3 at 25°C）, 7mM MgCl2, 40mM KCl, 10mM DTT, 0.1mg/ml BSA, 0.5mM [3H]dTTP, 0.025mM oligo（dT）, 0.25mM poly（A） and 0.01% NP-40.
For product intended use please see Patents & Disclaimers tab.

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